MAMMALIAN CELL LINE FOR PROTEIN PRODUCTION AND LIBRARY GENERATION

World Intellectual Property Organization Patent

APP PUB NO WO-2017174329-A1
SERIAL NO

PCTEP2017056373

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Abstract

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According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

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Patent Owner(s)

Patent OwnerAddress
ETH ZUERICHCHZURICH

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Inventor(s)

Inventor Name Address
REDDY SAI STEINENTORBERG 18 4051 BASEL BASEL
KELTON WILLIAM BASEL
PAROLA CRISTINA BASEL
MASON DEREK BASEL
POGSON MARK 3122 SE 16TH STREET NORTH BEND WASHINGTON 98045 98045

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