LARGE SCALE PRODUCTION PROCESS FOR CAPPED AND UN-CAPPED ANTIBODY CYSTEINES AND THEIR USE IN THERAPEUTIC PROTEIN CONJUGATION

World Intellectual Property Organization Patent

APP PUB NO WO-2018146585-A1
SERIAL NO

PCTIB2018050638

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Abstract

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Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs).; conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.

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Patent Owner(s)

Patent OwnerAddress
PFIZERUS235 EAST 42ND STREET NEW YORK NY 10017

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Inventor(s)

Inventor Name Address
ZHONG XIAOTIAN C/O PFIZER INC 610 MAIN STREET CAMBRIDGE MASSACHUSETTS 02139 02139
PRASHAD AMARNAUTH SHASTRIE NEW CITY NY
GOMES JOSE MANUEL CHELMSFORD MA 01824
DUTTA KAUSHIK C/O PFIZER INC 401 N MIDDLETOWN ROAD PEARL RIVER NEW YORK 10965 10965
KOTCH FRANK W PEARL RIVER NEW YORK 10965
PATEL VIMALKUMAR B JERSEY CITY NEW JERSEY 07306
PROCOPIO-MELINO RENEE L NORTH ANDOVER MASSACHUSETTS 01845

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