Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
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United States of America Patent
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Nov 21, 1995
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N/A
app pub date -
Mar 9, 1990
filing date -
Mar 13, 1986
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Abstract
Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.
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