Method of culturing cells using highly gas saturated media
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United States of America Patent
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Nov 27, 1990
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N/A
app pub date -
Apr 28, 1988
filing date -
Apr 28, 1988
priority date (Note) -
Expired
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Abstract
A method of culturing cells by providing a highly gassed media on a continuous basis to cells grown in a hollow fiber cartridge includes entraining the media with gas above the gas solubility level of the media. The media is then permitted to rest in a substantially quiescent state so that non-solubilized gas leaves the media. The media is then pressurized prior to delivery to the hollow fibers sufficiently so that the pressure drop and/or flow disturbance in the cell culturing device does not decrease the gas solubility level of the media below the media's gas saturation point.
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