Methods, reagents and instrumentation for preparing impregnated tissue samples suitable for histopathological and molecular studies
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United States of America Patent
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Apr 22, 2014
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app pub date -
Jul 31, 2006
filing date -
Jul 29, 2005
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Abstract
A process and kit for the production of paraffin sections of biological tissue, especially for molecular pathology studies, is described. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. The method is uncomplicated, requires little hands-on time, is reproducible and is standardized thereby limiting cross-center pre-analytical variance. A bio-indicator system for measuring the degree of crosslinking is also disclosed.
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