Process of purifying angiogenesis inhibitors
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United States of America Patent
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Aug 3, 2004
Grant Date -
N/A
app pub date -
Nov 27, 2000
filing date -
May 28, 1998
priority date (Note) -
Expired
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Abstract
The present invention relates to process of purifying the angiogensis inhibitor proteins expressed in E. coli and to the use of recombinant kringle 1-3 (; greenstatin) purified by the above process as a angiogenesis inhibitor and an anticancer agent. Particularly, according to the process of purifying the protein, the angiogenesis inhibitor proteins overexpressed are solubilized, refolded, and purified as a pure and active form. The greenstatin purified by the process specifically suppresses endothelial cell proliferation, anaiogenesis, and the growth of lung cancer, skin cancer, and brain tumor. Therefore, the process of this invention is applicable to the mass-production of angiogenesis inhibitor proteins such as greenstatin which is useful for the treatment of glaucoma, retinopathy, and cancers.
First Claim
Family
- 15 United States
- 10 France
- 8 Japan
- 7 China
- 5 Korea
- 2 Other
Patent Owner(s)
- KOREA GREEN CROSS CORPORATION
International Classification(s)
Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Ahn, Byung Cheul | Kyonggi-do, KR | 1 | 2 |
Chang, Soo-Ik | Chungcheongbuk-do, KR | 4 | 8 |
Hong, Yong-Kil | Seoul, KR | 1 | 2 |
Joe, Young Ae | Seoul, KR | 4 | 3 |
Jung, Soo-Il | Kyonggi-do, KR | 1 | 2 |
Kim, Young jo | Kyonggi-do, KR | 39 | 413 |
Lee, Hyosil | Kyonggi-do, KR | 1 | 2 |
Lee, Jong Hyuk | Seoul, KR | 85 | 299 |
So, Seung Ho | Taejon-si, KR | 2 | 2 |
You, Weon Kyoo | Kyonggi-do, KR | 4 | 17 |
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