Thermally stable tryptophanase, process for producing the same, and thermally stable tryptophanase-producing microorganism
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United States of America Patent
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Aug 2, 1988
Grant Date -
N/A
app pub date -
Jun 6, 1986
filing date -
Jun 8, 1985
priority date (Note) -
Expired
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Abstract
A thermally stable tryptophanase having (1) an optimum temperature for activity at a pH of 8.0 of about 70.degree. C., and (2) such thermal stability that it is not thermally deactivated when maintained at temperatures up to about 65.degree. C. and a pH of 8.0 for 40 minutes. The thermally stable tryptophanase can be produced by cultivating in a tryptophan-containing culture medium a thermally stable tryptophanase-producing bacterium which does not grow alone in said medium but grows there in the presence of Bacillus sp. strain S, and obtaining the resulting thermally stable tryptophanase from the culture broth. The thermally stable tryptophanase-producing microorganism for use in the above process is a novel organism.
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